Composite

Part:BBa_K4323003:Design

Designed by: Jessica Marinelli, Kevin Lim, Chun Hin Wong   Group: iGEM22_UManitoba   (2022-09-30)


Construct to express truncated FitD toxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 959
    Illegal BamHI site found at 1069
    Illegal BamHI site found at 2618
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2241
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We choose suitable promoters, RBS, and terminator to design this part. Between the RBS and the Coding region is a custom scar sequence (TACTAT).


Source

This composite part is synthesized and cloned by the 2022 UManitoba team

References